Biochemical and molecular characterization of Leishmania parasites isolated from an endemic focus in eastern Sudan
✍ Scribed by L. Oskam; F. Pratlong; E.E. Zijlstra; C.C.M. Kroon; J.-P. Dedet; P.A. Kager; G. Schönian; H.W. Ghalib; A.M. El-Hassan; S.E.O. Meredith
- Publisher
- Elsevier Science
- Year
- 1998
- Tongue
- English
- Weight
- 873 KB
- Volume
- 92
- Category
- Article
- ISSN
- 0035-9203
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✦ Synopsis
Twelve Leishmania isolates from visceral leishmaniasis patients in eastern Sudan were characterized using isoenzyme analysis, Southern blotting and polymerase chain reaction (PCR) 'fingerprinting'.
Isoenzyme analysis revealed the presence of 3 zymodemes: MON-18, MON-30 and MON-82, corresponding to Leishmania donovani sensu stricto, L. infantum and L. archibaldi (still of uncertain taxonomic status), respectively. Southern blotting and PCR 'fingerprinting' revealed identical patterns for all 3 zymodemes, which were indistinguishable from those of L. donovani s. s.