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Biochemical and kinetic characterization of the digestive trypsin-like activity of the lesser grain borer Rhyzopertha dominica (F.) (Coleoptera: Bostrichidae)

✍ Scribed by Pablo S. Osuna-Amarillas; Francisco J. Cinco-Moroyoqui; José L. Cárdenas-López; Josafat M. Ezquerra-Brauer; Rogerio Sotelo-Mundo; Mario O. Cortez-Rocha; Jesús M. Barrón-Hoyos; Ofelia Rouzaud-Sández; Jesús Borboa-Flores


Book ID
118481252
Publisher
Elsevier Science
Year
2012
Tongue
English
Weight
460 KB
Volume
51
Category
Article
ISSN
0022-474X

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A cDNA encoding a chymotrypsinogen-like protein in midguts of the lesser grain borer, Rhyzopertha dominica (F.) (Coleoptera: Bostrichidae) was cloned and sequenced. The 901 bp cDNA contains an 816-nucleotide open reading frame encoding 272-amino acids. The predicted molecular mass and pI of the matu