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Biochemical and immunological studies of three genetic variants of 3-phosphoglycerate kinase 2 from the mouse

✍ Scribed by Chi-Yu Lee; Bruna Pegoraro

Publisher: Springer
Year: 1979
Tongue: English
Weight: 666 KB
Volume: 17
Category: Article
ISSN: 0006-2928
DOI: 10.1007/bf00502123

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✦ Synopsis

Three electrophoretic variants of 3-phosphoglycerate kinase 2 (PGK-2A,PGK-2B, and PGK-2C) were purified from DBA/2J, C3H/HeJ, and C57L/J mice, respectively. PGK-2C exhibits only 2% of the specific activity of PGK-2A and PGK-2B in the reaction leading to the formation of 1,3-diphosphoglycerate. Compared to PGK-2A and PGK-2B, PGK-2C exhibits broader coenzyme specificity and lower Kms for substrate and coenzymes. Incubation at 45C revealed immunionactivation and double immunodiffusion studies showed that mice carrying any one of these three PGK-2 alleles have similar amounts of proteins for PGK-1 and PGK-2 in testes. The results of these studies suggest that low PGK-2C activity in C57L/J mice is a result of a structural rather than a regulatory gene mutation.

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