Biochemical and genetic analysis of an alpha-mannosidase mutant from Saccharomyces cerevisiae
β Scribed by Cueva, R
- Book ID
- 122134318
- Publisher
- John Wiley and Sons
- Year
- 1990
- Tongue
- English
- Weight
- 212 KB
- Volume
- 69
- Category
- Article
- ISSN
- 0378-1097
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Mutants of Saccharomyces cerevisiae lacking pyruvate kinase (EC 2.7.1.40) are described. These have less than 0.5% of the pyruvate kinase activity of the wild type. All the other glycolytic enzymes are present in normal amounts in these mutants. The mutation is recessive and segregates in diploids a
Sporulation-deficient mutants were isolated from a homothallic strain of Saccharomyces cerevisiae. Sporulation was induced in these mutants by procedures to sporulate the products of protoplast fusion between mutants and wild-type strains. Spores formed in this way were crossed to wild-type strains