Biochemical and functional analysis of mice deficient in expression of the CD45-associated phosphoprotein LPAP

✍ Scribed by Isabelle Ding; Eddy Bruyns; Ping Li; Diane Magada; Michael Paskind; Laura Rodman; Tara Seshadri; Denis Alexander; Thomas Giese; Burkhart Schraven

• Publisher: John Wiley and Sons
• Year: 1999
• Tongue: English
• Weight: 121 KB
• Volume: 29
• Category: Article
• ISSN: 0014-2980
• DOI: 10.1002/(sici)1521-4141(199912)29:12<3956::aid-immu3956>3.0.co;2-g

✦ Synopsis

The role of the CD45-associated phosphoprotein (LPAP/CD45-AP) during an immune response remains unclear. To understand better the function of LPAP we generated LPAPdeficient mice by disrupting exon 2 of the LPAP gene. LPAP-null mice were healthy and did not show gross abnormalities compared to their wild-type littermates. However, immunofluorescence analysis of T and B lymphocytes revealed a reduced expression of CD45, which did not affect a particular subpopulation. In contrast to a recent report (Matsuda et al., J. Exp. Med. 1998. 187: 1863-1870) we neither observed significant alterations of the assembly of the CD45/lck-complex nor of polyclonal T-cell responses. However, lymph nodes from LPAP-null mice showed increased cellularity, which could indicate that expression of LPAP might be required to prevent expansion of lymphocytes in particular lymphatic organs rather than potentiating immune responses.