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Biocatalytic properties of native and immobilized subtilisin 72 in aqueous-organic and low water media

โœ Scribed by Anna V. Bacheva; Anastasiya V. Belyaeva; Elena N. Lysogorskaya; Elena S. Oksenoit; Vladimir I. Lozinsky; Irina Yu. Filippova

Book ID
113803898
Publisher
Elsevier Science
Year
2005
Tongue
English
Weight
207 KB
Volume
32
Category
Article
ISSN
1381-1177

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Kinetic comparisons have been made between a randomly immobilized and a site-specifically immobilized subtilisin BPNะˆ on microfiltration membranes of varying hydrophilicities in both aqueous and organic media. Site-directed mutagenesis was employed to introduce a single cysteine into the amino acid