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Bioassay of retinoids using cultured human conjunctival keratinocytes

✍ Scribed by Brian M. Gilfix; Howard Green

Publisher
John Wiley and Sons
Year
1984
Tongue
English
Weight
304 KB
Volume
119
Category
Article
ISSN
0021-9541

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✦ Synopsis

The biological action of retinoids has been assayed using the differentiated properties of cultured human conjunctival keratinocytes. The effects measured were the suppression of envelope cross-linking and the promotion of synthesis of a keratin of molecular weight 40,000. Among the retinoids tested, the most powerful was the arotinoid Ro 13-6298, which reduced envelope formation detectably at 10(-11) M and by 90% at a concentration of 2 X 10(-10) M. The arotinoid was about 15 times more potent than trans-retinoic acid. The order of effectiveness of the retinoids in suppressing envelope cross-linking was the same as the order of effectiveness in promoting the synthesis of the 40-kd keratin.

πŸ“œ SIMILAR VOLUMES

Metabolism of an aromatic retinoid Ro 10
Metabolism of an aromatic retinoid Ro 10-9359 by cultured human epidermal keratinocytes
✍ Yukio Kitano; Natsuko Okada; Naoko Nagase πŸ“‚ Article πŸ“… 1982 πŸ› Springer-Verlag 🌐 English βš– 272 KB

An aromatic retinoid Ro 10-9359 is metabolized after absorption from intestine to form Ro 10-1670 which is an active therapeutic compound. The epidermal keratinocytes, a main target tissue of retinoid therapy in dermatology, was examined in the capacity to metabolize the retinoid. The culture of hum