Bioactive surface for neural electrodes: Decreasing astrocyte proliferation via transforming growth factor-β1
✍ Scribed by Christopher L. Klaver; Michael R. Caplan
- Publisher
- John Wiley and Sons
- Year
- 2007
- Tongue
- English
- Weight
- 184 KB
- Volume
- 81A
- Category
- Article
- ISSN
- 1549-3296
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✦ Synopsis
Abstract
Implantation of deep‐brain recording devices is a traumatic event, which inevitably elicits reactive gliosis. The ensuing glial scar encapsulating the implanted device impedes the long‐term functional recording capability of the microelectrode. In this work, a bioactive surface is prepared by conjugation of transforming growth factor‐beta one (TGF‐β1) and laminin to dextran, which is in turn conjugated to a biomaterial substrate. Poly‐L‐lysine coated surfaces are treated with oxidized dextran, and the dextran is re‐oxidized with sodium metaperiodate to generate hemiacetal structures to which TGF‐β1 and laminin are covalently bound. Covalent conjugation of the ligand is confirmed by enzyme‐linked immunosorbent assay. A primary cell line of astrocytes is incubated on a surface conjugated with laminin and TGF‐β1 and a surface only conjugated with laminin. Proliferation on the laminin plus TGF‐β1 surface is 57% less (p < 0.002) than the control surface (laminin alone). The results demonstrate that conjugated TGF‐β1 retains its efficacy toward astrocyte proliferation and represents a potential strategy for reducing glial scar formation in vivo. © 2007 Wiley Periodicals, Inc. J Biomed Mater Res, 2007