Bioactive substances from lespedeza cuneata L.G. Don and their biological activities

✍ Scribed by Hideyuki Shigemori; Naomi Sakai; Eiichi Miyoshi; Yoshikazu Shizuri; Shosuke Yamamura

• Book ID: 104203285
• Publisher: Elsevier Science
• Year: 1990
• Tongue: French
• Weight: 715 KB
• Volume: 46
• Category: Article
• ISSN: 0040-4020
• DOI: 10.1016/s0040-4020(01)85423-5

✦ Synopsis

Potassium lespedezate (3) and potassium isolespedezate (1) were isolated from the nyctinastic plant Lespedeza cuneata L. G. Don. These compounds (3 and 4 effective for leaf-opening of the plant Cassia mimosoides L. at 8x10-$ were quite mol/l. The interaction between the leaf-closing substance (2) and leaf-opening substances (3 and 4) shall govern in circadian rhythm of Cassia plants. 384 H. SHIOEMORI et al. of Mimosa pudica. Our Mimosa test is quite similar to the Fitting-Hess-Schildknecht test using the leaves of the plant, lv3) which shows a rapid response, but must be carefully carried out because it is quite sensitive to H+ ion [the leaves were closed on addition of dil. H2S04 (10m4 mol/l)]. temperature, humidity, and others. In bioassay of leaf-opening factor, we have used the leaves of Cassia mimosoides L. The young leaves to be tested have been immersed in distilled water and allowed to stand at room temperature for several hours. As usual, the leaves closed at night. However, when the bioactive principles were contained in the test solution, the leaves opened again even at night. The Cassia test is quite