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Bio-orthogonal Affinity Purification of Direct Kinase Substrates

✍ Scribed by Allen, Jasmina J.; Lazerwith, Scott E.; Shokat, Kevan M.


Book ID
125941418
Publisher
American Chemical Society
Year
2005
Tongue
English
Weight
87 KB
Volume
127
Category
Article
ISSN
0002-7863

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The ability of protein kinases to phosphorylate synthetic peptides corresponding to identified protein phosphorylation sites has previously been used to determine primary structural requirements and has helped define distinct "recognition sequences" for a variety of enzymes. Here, we have used an im