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Binding properties of acetylcholine receptors extracted from normal and from denervated rat diaphragm

โœ Scribed by Richard Alper; Joseph Lowy; Jakob Schmidt


Book ID
115905004
Publisher
Elsevier Science
Year
1974
Tongue
English
Weight
257 KB
Volume
48
Category
Article
ISSN
0014-5793

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The acetylcholine receptor protein from human muscle was extracted with the non-ionic detergent Triton X-100 and purified by affinity chromatography on a-Naja toxin sepharose 4B. Further purification on Dicap-MP sepharose 4B, a choline analog compound, led to ACHR preparations with specific activiti