Binding of wheat and peanut lectins to human transitional cell carcinomas. Correlation with histopathologic grade, invasion, and dna ploidy
✍ Scribed by Niels C. Langkilde; Hans Wolf; Torben F. Ørntofty
- Publisher
- John Wiley and Sons
- Year
- 1989
- Tongue
- English
- Weight
- 530 KB
- Volume
- 64
- Category
- Article
- ISSN
- 0008-543X
No coin nor oath required. For personal study only.
✦ Synopsis
The binding of peanut (PNA) and wheat germ (WGA) lectins to tissue sections was examined in biopsy specimens from normal urothelium (ten patients) and from tumor tissue of noninvasive (I 7 patients) and invasive bladder (31 patients) carcinomas. The results were correlated to DNA content, histopathologic grade, and the presense or absence of invasion. Significant alterations in lectin binding associated with the development of cancer were found. A gradual loss of both PNA and WGA binding was found to correlate with higher grades of atypia (P < 0.001). The loss of WGA binding was significantly correlated with both tumor aneuploidy (P < 0.001) and the presence of invasion (P < 0.05), whereas no significant correlation was found between loss of PNA binding and these variables. We concluded that the loss of WGA binding structures associated with bladder cancer shows a better correlation with known risk factors (aneuploidy and invasion) than the loss of PNA binding does.
Cancer 64:849-853, 1989.
HE GREAT VARIATION in the biologic behavior of T bladder carcinomas presents a clinical problem.
Many patients with low-grade tumors will have recurrence of their tumor and others, although only a few, will experience progression to invasive disease. Unfortunately, the information available from histopathologic evaluation does not allow for the identification of the tumors possessing the biologic potential for rapid progression.'** Recently, new methods have been investigated in the search for clinically significant markers and predictors of tumor recurrence and tumor progression. Special interest has been given to changes in the expression of the T-anti-gen3-' The altered pattern of T-antigen expression in bladder cancer has been proposed as a useful marker because changes in T-antigen expression seem to correlate with subsequent invasion and Measurements of the DNA content of cells by flow cytometry has received considerable interest, and several authors have shown that aneuploid DNA content of urothelial tumor cells is related to invasive disease.'-I4
Previous immunohistochemical studies have shown