Binding of sulfisoxazole to protein fractions of tears

✍ Scribed by Sukhbir S. Chrai; Joseph R. Robinson

• Publisher: John Wiley and Sons
• Year: 1976
• Tongue: English
• Weight: 364 KB
• Volume: 65
• Category: Article
• ISSN: 0022-3549
• DOI: 10.1002/jps.2600650333

✦ Synopsis

By using equilibrium dialysis experiments, it is shown that sulfisoxazole binds extensively with albumin and a-globulin and to a lesser extent with y-globulin and lysozyme, all of which are normal components of tears. The binding of sulfisoxazole to the various protein fractions quantitatively accounts for the overall binding of this drug to human tears. It is suggested that to predict the binding of drugs to proteins in lacrimal fluid, both in normal and pathological conditions, it is necessary to conduct binding studies of the drug with the major protein fractions of tears. Moreover, it is essential to know the composition of the major protein fractions of tears in both normal and various pathological conditions to conduct the necessary in uitro binding studies.

Keyphrases Sulfisoxazole-binding to protein fractions of human tears, normal and irritant induced Protein binding-sulfisoxazole to protein fractions of human tears, normal and irritant induced Lacrimal fluids-human, normal and irritant induced, binding of sulfisoxazole to protein fractions ' All spectral measurements were carried out on either a Cary 14 or Cary * Union Carbide Corp., Chicago, Ill.

SchwardMann, Orangeburg, N.Y. A unit is defined as a decrease in absorbance a t 450 nm of 0.001/min at