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Binding of RFX2 and NF-Y to the testis-specific histone H1t promoter may be required for transcriptional activation in primary spermatocytes

✍ Scribed by Jane M. vanWert; Steven A. Wolfe; Sidney R. Grimes

Publisher
John Wiley and Sons
Year
2008
Tongue
English
Weight
371 KB
Volume
104
Category
Article
ISSN
0730-2312

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✦ Synopsis

Abstract

The testis‐specific linker histone H1t is transcribed exclusively in pachytene spermatocytes during spermatogenesis. The H1t promoter contains two imperfect inverted repeats which together comprise the X‐box motif that is known to bind the transcription factor regulatory factor X (RFX). Out of all the histone H1 family promoters this motif appears only in the H1t promoter and may contribute to H1t tissue‐specific expression. We show by Western blotting, EMSA, ChIP analysis, and real‐time RT‐PCR that the rat H1t X‐box is bound by RFX2 in vivo in spermatocytes. We demonstrate that transcription factor NF‐Y binds to the CCAAT‐box motif that is located downstream and adjacent to the X‐box and that testis NF‐Y interacts either directly or indirectly with RFX2. Furthermore, we show that both the X‐box and CCAAT‐box are required for promoter activity and that co‐expression of RFX2 greatly enhances testis histone H1t promoter activity in the GC‐1spg germinal cell line. J. Cell. Biochem. 104: 1087–1101, 2008. © 2008 Wiley‐Liss, Inc.

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