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Binding of quinacrine to the human Y chromosome

✍ Scribed by J. M. R. Hatfield; K. W. C. Peden; R. M. West


Publisher
Springer
Year
1975
Tongue
English
Weight
698 KB
Volume
52
Category
Article
ISSN
0009-5915

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✦ Synopsis


Human chromosome spreads were stained with 3H-quinacrine and their fluorescence observed. The exact location of specific spreads on each slide was noted and photographs taken. Autoradiographs were then prepared so that the quinacrine fluorescence of any specific chromosome could be compared directly with the distribution of grains over the same chromosome on the autoradiograph. The Y chromosome fluoresced much more intensely than any of the other chromosomes, but there were no more grains over the Y chromosome than over the other chromosomes. Therefore the enhanced fluorescence of the human Y chromosome is not due to an increased binding of quinacrine.


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A karyotype analysis of a diploid inbred line of Petunia hybrida stained with aceto-orcein is given. Five of the seven pairs of chromosomes can be identified by their relative lengths and arm ratios. The two remaining pairs stained with quinacrine show different fluorescence patterns.