Binding of puromycin toE. coliribosomes. Effects of puromycin analogues and peptide bond formation inhibitors

✍ Scribed by Rafael Fernandez-Muñoz; David Vazquez

• Book ID: 104738497
• Publisher: Springer
• Year: 1973
• Tongue: English
• Weight: 309 KB
• Volume: 1
• Category: Article
• ISSN: 0301-4851
• DOI: 10.1007/bf00357402

✦ Synopsis

aH-puromycin binds to bacterial ribosomes, in the presence of ethanol, under the experimental conditions of the fragment reaction assay. The binding is feeble, takes place at 0~ is partially inhibited by chloramphenicol and lincomycin and totally by sparsomycin. 3H-puromycin binding is hardly affected by the 3' aminonucleoside of puromycin, is well inhibited by the L-Phenylalanine and L-Leucine analogues of puromycin and totally blocked by L-Phenylalanyladenosine.