Binding of purine nucleotides to ammonium formate during gel filtration on Sephadex G-10

✍ Scribed by Carl Bernofsky

• Book ID: 102628130
• Publisher: Elsevier Science
• Year: 1975
• Tongue: English
• Weight: 205 KB
• Volume: 68
• Category: Article
• ISSN: 0003-2697
• DOI: 10.1016/0003-2697(75)90709-5

✦ Synopsis

Gel filtration of a mixture of ATP and ammonium formate on Sephadex G-10 gives rise to two peaks of ATP. The first peak is eluted near the void volume, while the second peak is eluted together with the ammonium formate. More than 50% of the ATP can be present in the second peak, depending on the concentration of ammonium formate and the degree of compression of the gel bed. ATP appears to partition into the salt peak via hydrogen bonding to ammonium formate. Similar results are obtained with GTP but not with ITP.