Binding of Proflavine to α-Chymotrypsin and Trypsin and Its Displacement by Avian Ovomucoids *
✍ Scribed by Feinstein, Gad; Feeney, Robert E.
- Book ID
- 127106401
- Publisher
- American Chemical Society
- Year
- 1967
- Tongue
- English
- Weight
- 1023 KB
- Volume
- 6
- Category
- Article
- ISSN
- 0006-2960
No coin nor oath required. For personal study only.
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## Abstract The effect of pH and temperature on the apparent association equilibrium constant (__K__~a~) for the binding of the soybean Bowman‐Birk proteinase inhibitor (BBI) and of its chymotrypsin and trypsin inhibiting fragments (F‐C(p), F‐T(p) and F‐T(t), respectively) to bovine α‐chymotrypsin
Development of a simple and efficient purification procedure for Shiga-like toxin I (Stx1) was attempted. Since it has been suggested that pigeon egg white ovomucoid carries a P1 antigenic determinant, we examined its ability to bind Stx1. The ovomucoid glycoprotein fraction (GPro) was prepared fro