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Binding of periodate-oxidized guanine nucleotides to eukaryotic elongation factor 2

✍ Scribed by Neşe Bilgin; Osman Sayhan; Engin Bermek

Book ID
119119860
Publisher
Elsevier Science
Year
1990
Tongue
English
Weight
605 KB
Volume
1048
Category
Article
ISSN
0167-4781

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Labeling of Adenine and Guanine Nucleotide-Binding Proteins in Permeabilized Cells with in Situ Periodate-Oxidized Nucleotides
✍ M.E. Peter; J. She; L.A. Huber; C. Terhorst 📂 Article 📅 1993 🏛 Elsevier Science 🌐 English ⚖ 1019 KB

A novel approach for identification of adenine and guanine nucleotide-binding proteins in permeabilized cells is described. Cells were incubated for various periods with \(\alpha-{ }^{32} \mathrm{P}\)-labeled nucleotides and then briefly treated with periodate. Condensation products formed in situ b