𝔖 Bobbio Scriptorium
✦   LIBER   ✦

Binding of metabolites of dietary 4-dimethyl-aminoazobenzene and 2-methyl-4-dimethylamino-azobenzene to rat liver DNA and protein of subcellular fractions

✍ Scribed by Jean Chauveau; Michèle Meunier; Annie Benoit

Publisher
John Wiley and Sons
Year
1974
Tongue
French
Weight
553 KB
Volume
13
Category
Article
ISSN
0020-7136

No coin nor oath required. For personal study only.

✦ Synopsis

Abstract

The binding of metabolites of two related azo dyes of different carcinogenic potency, the carcinogenic 4‐dimethylaminoazobenzene (DAB) and the weakly carcinogenic 2‐methyl‐4‐dimethylaminoazobenzene (2‐Me‐DAB), to rat liver DNA and to subcellular fraction protein was studied following chronic oral administration for 1 to 3 weeks. Different techniques for measuring the amount of DAB metabolites bound to protein were first compared, then the whole study was performed with labelled DAB and 2‐Me‐DAB (aniline ring‐^14^C) of moderate specific activity. DAB metabolites were bound to liver DNA to a higher extent than those of 2‐Me‐DAB. In contrast, the binding of 2‐Me‐DAB metabolites was equal to or higher than that of DAB metabolites to protein. The amount of protein‐bound metabolites was studied on the nucleo‐mito‐chondrial fraction, microsomes, supernatant, nuclei, chromatin, nucleoplasm, nucleolar fraction and nuclear membrane. Following the administration of both dye diets, the supernatant protein bound the highest level of metabolites. The time‐course of binding of DAB metabolites to DNA and protein was different from that of 2‐Me‐DAB metabolites. These results show the possible involvement of carcinogen‐DNA binding in the mechanism of carcinogenesis.

📜 SIMILAR VOLUMES

Binding of a metabolite of triflusal (2-
Binding of a metabolite of triflusal (2-hydroxy-4-trifluoromethylbenzoic acid) to serum proteins in rat and man
✍ R. Mis; J. Ramis; L. Conte; J. Forn 📂 Article 📅 1992 🏛 Springer 🌐 English ⚖ 456 KB

2-hydroxy-4-trifluoromethylbenzoic acid (HTB) is the main active metabolite of the platelet antiaggregant drug triflusal. Its binding to plasma proteins of rats and healthy volunteers in vitro and in vivo has been studied. Rats were given a single oral dose of 50 mg.kg-1 triflusal and the healthy vo

The binding of reactive metabolites of t
The binding of reactive metabolites of the carcinogenN-hydroxy-2-acetylaminofluorene to DNA and protein in isolated rat liver nuclei: Effects of glutathione and methionine
✍ Meerman, John H. N. ;Tijdens, Roeline B. ;Mulder, Gerard J. 📂 Article 📅 1986 🏛 John Wiley and Sons 🌐 English ⚖ 580 KB

The covalent binding of reactive metabolites of the carcinogen N-hydroxy-2-acetylaminofluorene to DNA and protein in isolated, intact rat liver nuclei was studied. The chemically synthesized 2-acetylaminofluorene-N-sulfate became covalently bound to DNA and protein to form adducts, 50% to 60% of whi