Binding of lanthanum and gadolinium ions to Concanavalin A studied calorimetrically at 25°C

✍ Scribed by Guido Barone; Giuseppina Castronuovo; Pompea Del Vecchio; Vittorio Elia; Patrizia Guarrata

• Publisher: John Wiley and Sons
• Year: 1989
• Tongue: English
• Weight: 518 KB
• Volume: 2
• Category: Article
• ISSN: 0952-3499
• DOI: 10.1002/jmr.300020402

✦ Synopsis

The interaction between Concanavalin A (ConA) and the lanthanide ions La3+ and Gd3+ has been studied calorimetrically a t 25 O C . The measurements were carried out a t a pH of 4.5, where the protein exists prevailingly as a dimer. Calorimetry allows the direct determination of the binding enthalpy and the evaluation of both the apparent association constant, and the apparent free energy and entropy. Three groups of data were collected. The first concerns the interaction of the 'native' protein, i.e., fully metallized with Mn2+ and Ca2+, with the lanthanides. The second concerns the interaction of the completely demetallized protein with La3+ and Gd3+. Finally, the affinity of each complex was tested for the specific sugar a-methylmannopyranoside. The analysis of the thermodynamic parameters obtained, led to the following conclusions: 1) a specific site, named S3, exists on the protein for the lanthanides, distinct from the S1 site of the transition metal and from the S2 site, specific for calcium. There is only one S3 site per protomer when the protein has Mnz+ in S1 and Ca2+ in S2. Moreover, there is no appreciable competition for S1 and S2 from the lanthanides. The 'native' protein, metallized with La3+ or Gd3+, is a fully functional protein.

2. The demetallized protein (ApoCon A) has at least two sites per protomer for the lanthanides. The hypothesis is that, besides the S3 site, the lanthanides, in the absence of Mn", can also occupy the S1, but not the S2, site. The protein metallized only with gadolinium ion is completely inactive toward the interaction with the mannoside. The same happens when, along with gadolinium, only calcium or manganese is present. Hence, in the absence of the transition metal in S1 or of calcium in S2, the protein is not in the conformation suitable to interact with its specific substrate.