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Binding of hirudin to human α, β and γ-thrombin: A comparative kinetic and thermodynamic study

✍ Scribed by Paolo Ascenzi; Gino Amiconi; Massimo Coletta; Giulio Lupidi; Enea Menegatti; Silvia Onesti; Martino Bolognesi


Book ID
115985813
Publisher
Elsevier Science
Year
1992
Tongue
English
Weight
965 KB
Volume
225
Category
Article
ISSN
0022-2836

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The binding of a series of low-molecular-mass, active-site-directed thrombin inhibitors (399 -575 Da) to human ␣-thrombin was investigated by surface plasmon resonance technology (BIACORE), stopped-flow spectrophotometry, and isothermal titration microcalorimetry (ITC). The equilibrium constants K D