Binding of fibrinogen molecules to pig platelets and their membranes
β Scribed by CIERNIEWSKI, C; KOWALSKA, M; KRAJEWSKI, T; JANIAK, A
- Book ID
- 123347481
- Publisher
- Elsevier Science
- Year
- 1982
- Tongue
- English
- Weight
- 418 KB
- Volume
- 714
- Category
- Article
- ISSN
- 0304-4165
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Dog and human fibrinogen were derivatized with N-hydroxysuccinimidofluorescein and utilized for flow cytometric estimation of fibrinogen binding to activated platelets. Fluorescein-fibrinogen binding fulfilled the criteria for specific binding to platelets; the binding was saturable, dependent on ag
Arg-Gly-Asp (RGD)-containing peptides and the peptide unique to fibrinogen in the C-terminal domain of the gamma chain are important for fibrinogen binding to platelet membrane glycoprotein (GP) II b/III a. We synthesized a unique hybrid peptide of YRGDSPLGGAKQAGDV encompassing the RGD sequence (RGD