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Binding of ethidium to bacteriophage T7 and T7 deletion mutants

✍ Scribed by Gary A. Griess; Philip Serwer; Paul M. Horowitz


Publisher
Wiley (John Wiley & Sons)
Year
1985
Tongue
English
Weight
637 KB
Volume
24
Category
Article
ISSN
0006-3525

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Kinetics of ethidium's intercalation in
✍ Gary A. Griess; Philip Serwer; Varsha Kaushal; Paul M. Horowitz πŸ“‚ Article πŸ“… 1986 πŸ› Wiley (John Wiley & Sons) 🌐 English βš– 653 KB

The kinetics of ethidium's intercalative binding to DNA packaged in bacteriophage T7 and two T7 deletion mutants have been determined, using enhancement of fluorescence to quantitate binding. At a constant ethidium concentration. the results can be described as first-order binding with two different

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## Abstract We have measured the kinetics of proflavin binding to T‐even bacteriophagesβ€”the 700 S and 1000 S forms of T2L, T4D, and T4D os41β€”by difference spectroscopy at 430 nm. Measurements were carried out from 22Β° to 37Β°C. Binding is very slow to encapsulated DNA compared to free DNA, requiring

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The structure of the packaged double-stranded DNA genome of bacteriophage T7 was compared to that of unpackaged T7 DNA using digital difference Raman spectroscopy. Spectral data were obtained at 25 degrees C from native T7 virus (100 mg/mL), empty T7 capsids (50 mg/mL), and purified T7 DNA (40 mg/mL