Binding of ethidium to bacteriophage T7 and T7 deletion mutants
β Scribed by Gary A. Griess; Philip Serwer; Paul M. Horowitz
- Publisher
- Wiley (John Wiley & Sons)
- Year
- 1985
- Tongue
- English
- Weight
- 637 KB
- Volume
- 24
- Category
- Article
- ISSN
- 0006-3525
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π SIMILAR VOLUMES
The kinetics of ethidium's intercalative binding to DNA packaged in bacteriophage T7 and two T7 deletion mutants have been determined, using enhancement of fluorescence to quantitate binding. At a constant ethidium concentration. the results can be described as first-order binding with two different
## Abstract We have measured the kinetics of proflavin binding to Tβeven bacteriophagesβthe 700 S and 1000 S forms of T2L, T4D, and T4D os41βby difference spectroscopy at 430 nm. Measurements were carried out from 22Β° to 37Β°C. Binding is very slow to encapsulated DNA compared to free DNA, requiring
## Abstract We have measured the binding equilibria of proflavin to T2L bacteriophage, in both βslowβ and βfastβ sedimenting forms, and to free T2L DNA. Measurements were carried out by difference spectroscopy at 430 nm at temperatures from 13 to 43Β°C and at pH 5.6 and 7.6. We found no significant
The structure of the packaged double-stranded DNA genome of bacteriophage T7 was compared to that of unpackaged T7 DNA using digital difference Raman spectroscopy. Spectral data were obtained at 25 degrees C from native T7 virus (100 mg/mL), empty T7 capsids (50 mg/mL), and purified T7 DNA (40 mg/mL