Binding of epidermal growth factor by human colon carcinoma cell (Caco-2) monolayers
β Scribed by Ismael J. Hidalgo; Akira Kato; Ronald T. Borchardt
- Book ID
- 115761908
- Publisher
- Elsevier Science
- Year
- 1989
- Tongue
- English
- Weight
- 456 KB
- Volume
- 160
- Category
- Article
- ISSN
- 0006-291X
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## Abstract Human colon cancer cell lines express epidermal growth factor (EGF) mRNA, secrete EGF and may respond to it __via__ the cellβsurface EGF receptor (EGFR). Expression of these molecules in human colon and colon tumor, however, is not clear. Reverse transcriptionβpolymerase chain reaction
The functional role of epidermal growth factor (EGF) in epithelium-derived human colonic carcinoma cells was investigated by transfection with plasmid pUCDS3, which contained synthetic human EGF encoding sequences, into two human colonic carcinoma cell types with dissimilar phenotypic properties: th