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Binding of aminoacyl-tRNA to rat liver ribosomal proteins

✍ Scribed by Ramon Reyes; Luis Carrasco; David Vazquez

Publisher
Springer
Year
1976
Tongue
English
Weight
272 KB
Volume
2
Category
Article
ISSN
0301-4851

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✦ Synopsis

Rat liver ribosome treatment with ethanol and 1 M NH4Cl releases some 31-33 ribosomal proteins. This split protein fraction binds Phe-tRNA, Ac-Phe-tRNA, Met-tRNAM and f-Met-tRNAF in the absence of K+ and Mg++ ions. When the split protein fraction is passed through Sephadex G-100 only six proteins are retained in the column: S10, S14, S15, S19, L35, and L36. The aminoacyl-tRNA binding activity of this protein fraction retained in the Sephadex G-100 column is similar to that of the total split protein fraction, suggesting that the above six proteins, or only some of them, are involved in the binding reaction.

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## Abstract Ricin A chain was radioactively labeled using reductive alkylation, lactoperoxidase catalyzed iodination, and reaction with iodoacetamide or N‐ethylmaleimide (NEM). The inhibition of cell‐free rat liver protein synthesis by the modified A chains and the ribosome binding characteristics