Binding of alkyl 1-Thio-β-d-galactopyranosides to β-d-galactosidase from E. coli
✍ Scribed by Clement K. De Bruyne; Marc Yde
- Book ID
- 108308493
- Publisher
- Elsevier Science
- Year
- 1977
- Tongue
- English
- Weight
- 899 KB
- Volume
- 56
- Category
- Article
- ISSN
- 0008-6215
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📜 SIMILAR VOLUMES
The enzyme-catalyzed hydration2 of D-galactal and the enzyme-catalyzed hydrolysis of fl-D-galactopyranosides are similar as far as the products are concerned. In the case of hydration of D-galactal, a covalent, 2deoxy-D-Zyxo-hexosy1("2deoxy-D-galactosyl")enzyme intermediate could be isolated' . As s
Different ethyl 1-thio-1]-D-disaccharides have been synthesised by transgalactosylation using the 1]-gaiactosidase from Bacillus circulans as biocatalyst. This [~-
A value of 1.8 has been determined for (K I ) HOH /(K I ) DOD , the ratio of the values of K I for competitive inhibition of β-galactosidase by isopropyl β-D-thiogalactopyranoside in H 2 O and D 2 O. This is similar to the value of 1.7 for (K m ) HOH /(K m ) DOD , the ratio of the Michaelis constant