Binding of 125I-iodovinyltetrabenazine to CNS vesicular monoamine transport sites

✍ Scribed by Mei-Ping Kung; Daniel J. Canney; Dana Frederick; Zheng Zhuang; Jeffrey J. Billings; Hank F. Kung

• Book ID: 104600582
• Publisher: John Wiley and Sons
• Year: 1994
• Tongue: English
• Weight: 867 KB
• Volume: 18
• Category: Article
• ISSN: 0887-4476
• DOI: 10.1002/syn.890180308

✦ Synopsis

Binding characteristics of a novel radioiodinated tetrabenazine (TBZ) analog (iodovinyltetrabenazine; 1251-TBZ-Fraction I) were evaluated. In rat striatal homogenates, 1251-TBZ-I displayed a pharmacological profile consistent with specific binding to vesicular monoamine transport (VMAT) sites. In vitro autoradiographic studies using rat brain sections further demonstrated that lZ5I-TBZ-I labeled the regions rich in VMAT sites, and it may be a useful marker for these sites. This novel radioiodinated ligand, with high specific activity and high binding affinity, may provide a powerful tool for the in vitro assessment of neuronal loss in various neurodegenerative diseases.