Binding and functional characteristics of two E-box motifs within the S100A6 (calcyclin) gene promoter

✍ Scribed by Wiesława Leśniak; Jacek Kuźnicki

• Book ID: 102302667
• Publisher: John Wiley and Sons
• Year: 2006
• Tongue: English
• Weight: 143 KB
• Volume: 97
• Category: Article
• ISSN: 0730-2312
• DOI: 10.1002/jcb.20699

✦ Synopsis

Abstract

S100A6 (calcyclin) is a small calcium‐binding protein of the S100 family often associated with cancer and metastasis. We have previously shown that the E‐box sequence at position −283/−278 of the S100A6 gene promoter interacts with USF transcription factor and contributes to promoter transcriptional activity. We now present evidence that a second E‐box motif at position −593/−588 of the promoter also binds USF and that the USF1/USF2 heterodimer is the prevailing dimeric form of the transcription factor bound. Using the chromatin immunoprecipitation assay (ChIP), we show that USF is bound in vivo to the E‐box regulatory element(s). Depletion of the endogenous USF pool by means of a decoy oligonucleotide evokes a severe inhibition of S100A6 gene promoter activity. Furthermore, we show that S100A6 gene promoter activity can be stimulated by palmitate and that mutation of the −283/−278 E‐box sequence completely blocks this stimulation. J. Cell. Biochem. 97: 1017–1024, 2006. © 2005 Wiley‐Liss, Inc.