Bilirubin-displacing effect of ampicillin, indomethacin, chlorpromazine, gentamicin, and parabens in vitro and in newborn infants
✍ Scribed by Rolf Brodersen; Finn Ebbesen
- Publisher
- John Wiley and Sons
- Year
- 1983
- Tongue
- English
- Weight
- 716 KB
- Volume
- 72
- Category
- Article
- ISSN
- 0022-3549
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✦ Synopsis
Displacement of bilirubin bound to human serum albumin by ampicillin, indomethacin, chlorpromazine, gentamicin, methylparaben, and propylparaben was investigated quantitatively. Two methods were used in uitro: measurement of bilirubin displacement by studying the rate of bilirubin oxidation with hydrogen peroxide and peroxidase and determination of the albumin reserve for binding of bilirubin by observation of the dialysis rate of an added trace amount of a deputy ligand monoacetyldapsone (p-acetamido-p'-aminodiphenyl sulfone). The latter method was also used for the determination of the albumin reserve in sera from treated newborn infants. The following doses were given: ampicillin, 100 mg/kg iv; indomethacin, 0.2 mg/kg iv; chlorpromazine hydrochloride, 0.7 mg/kg im; gentamicin sulfate, 2.5 mg/kg im. The parabens were present in injectable preparations of chlorpromazine and gentamicin and were therefore given in the following doses: methylparaben, 0.35 mg/kg, and propylparaben, 0.05 mg/kg. All drugs were given in a single dose. A few additional additives and metabolites were studied in uitro. Ampicillin, given to 19 infants, produced a small, significant decrease in plasma albumin reserve, to 82% of the pretreatment level and, thus, had a slight bilirubin-displacing effect, quantitatively consistent with a weak displacing effect measured in uitro. None of the other substances showed any measurable displacement in uiuo, likewise in agreement with the results from in uitro studies.
Keyphrases 0 Bilirubin-displacing effect of ampicillin, indomethacin, chloropromazine, gentamicin, and parabens, in uitro and in newborn infants 0 Ampicillin-bilirubin-displacing effects of indomethacin, chlorpromazine, gentamicin, and parabens, in uitro and in newborn infants n Indomethacin-bilirubin-displacing effects of ampicillin, chlorpromazine, gentamicin, and parabens, in uitro and in newborn infants n Chlorpromazine-bilirubin-displacing effects of ampicillin, indomethacin, gentamicin, and parabens, in uitro and in newborn infants 0 Gentamicin-bilirubin-displacing effects of ampicillin, indomethacin, chlorpromazine, and parabens, in uitro and in newborn infants 0 Parabens-bilirubin-displacing effects of ampicillin, indomethacin, chlorpromazine, and gentamicin, in uitro and in newborn infants AB Kabi,