bFGF induces S1P1 receptor expression and functionality in human pulmonary artery smooth muscle cells

✍ Scribed by Magdalena Birker-Robaczewska; Rolf Studer; Bénédicte Haenig; Katalin Menyhart; Silke Hofmann; Oliver Nayler

• Publisher: John Wiley and Sons
• Year: 2008
• Tongue: English
• Weight: 204 KB
• Volume: 105
• Category: Article
• ISSN: 0730-2312
• DOI: 10.1002/jcb.21918

✦ Synopsis

Abstract

Sphingosine‐1‐phosphate (S1P), acting through five closely related G‐protein coupled receptors termed S1P~1–5~, has recently emerged as a possible regulator of smooth muscle cell (SMC) physiology with the potential to induce contraction, proliferation and stress fiber formation. In the present study, real‐time quantitative PCR was used to determine the expression patterns of S1P receptor subtypes in human primary pulmonary artery smooth muscle cells (PASMC). We report here that subconfluent PASMC express predominantly S1P~2~ and S1P~3~ receptors and we show that S1P~1~ receptor mRNA levels are significantly up‐regulated following basic fibroblast growth factor (bFGF) treatment. As a consequence, increased responsiveness, as measured by impedance and ERK1/2 phosphorylation, was observed upon stimulation with a specific S1P~1~ receptor agonist SEW2871. We therefore demonstrate, for the first time, that a growth factor that was previously shown to be involved in physiological and pathological changes of SMC function induced S1P~1~ receptor expression and we propose that S1P~1~ receptor up‐regulation could contribute to vascular remodeling. J. Cell. Biochem. 105: 1139–1145, 2008. © 2008 Wiley‐Liss, Inc.