Beyond DnaA: The Role of DNA Topology and DNA Methylation in Bacterial Replication Initiation
✍ Scribed by Donczew, Rafał; Zakrzewska-Czerwińska, Jolanta; Zawilak-Pawlik, Anna
- Book ID
- 121748866
- Publisher
- Elsevier Science
- Year
- 2014
- Tongue
- English
- Weight
- 846 KB
- Volume
- 426
- Category
- Article
- ISSN
- 0022-2836
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Flow cytometry was used to study initiation of DNA replication in Escherichia coli K12 after induced expression of a plasmid-borne dnaA+ gene. When the dnaA gene was induced from either the plac or the lambda pL promoter initiation was stimulated, as evidenced by an increase in the number of origins
## Abstract Genomic propagation in both prokaryotes and eukaryotes is tightly regulated at the level of initiation, ensuring that the genome is accurately replicated and equally segregated to the daughter cells. Even though replication origins and the proteins that bind onto them (initiator protein