Behavior of rat periodontal ligament cells on fibroblast growth factor-2-immobilized titanium surfaces treated by plasma modification
✍ Scribed by Eitoyo Kokubu; Masao Yoshinari; Kenichi Matsuzaka; Takashi Inoue
- Publisher
- John Wiley and Sons
- Year
- 2009
- Tongue
- English
- Weight
- 181 KB
- Volume
- 91A
- Category
- Article
- ISSN
- 1549-3296
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✦ Synopsis
Abstract
The aim of this study was to investigate the behavior of rat periodontal ligament (PDL) cells cultured on fibroblast growth factor‐2 (FGF‐2)‐immobilized titanium surfaces treated by oxygen (O~2~) plasma. We used cell disks (15 mm in diameter), and 35‐mm culture dishes sputter‐coated with titanium. These were treated with oxygen plasma and dipped in FGF‐2 solution. Immobilized FGF‐2 was visualized with a confocal laser‐scanning microscope, and its weight was calculated to be ˜22.6 ng/cm^2^ using a quartz crystal microbalance‐dissipation apparatus. The PDL cells were obtained from rat incisors. Cells from fourth subculture were seeded onto the FGF‐2‐immobilized titanium surface. Proliferation ratio, alkaline phosphatase (ALP) activity, and expressions of type I collagen and vascular endothelial growth factor (VEGF) mRNAs were evaluated. Proliferation ratio and expressions of type I collagen and VEGF mRNAs were significantly higher, whereas ALP activity was significantly lower in FGF‐2‐immobilized cells than in control group (p < 0.05). These findings suggest that oxygen plasma modification can immobilize FGF‐2 onto a titanium surface. Immobilized FGF‐2, although inferior to culture medium with FGF‐2, influenced the proliferation of PDL cells and might have promoted collagen and vascular synthesis. © 2008 Wiley Periodicals, Inc. J Biomed Mater Res 2009