Behavior of fetal rat chondrocytes cultured on a bioactive glass-ceramic

✍ Scribed by Loty, C. ;Forest, N. ;Boulekbache, H. ;Kokubo, T. ;Sautier, J. M.

• Publisher: John Wiley and Sons
• Year: 1997
• Tongue: English
• Weight: 345 KB
• Volume: 37
• Category: Article
• ISSN: 0021-9304
• DOI: 10.1002/(sici)1097-4636(199710)37:1<137::aid-jbm17>3.0.co;2-d

✦ Synopsis

We examined the behavior of fetal rat chon-the material, and, finally, on day 10, formed nodular drocytes cultured on a bioactive glass-ceramic containing structures composed of round cells separated by a dense apatite and wollastonite (A.W.G.C.). Biomaterial surface extracellular matrix. Furthermore, these clusters of round topography and profiles were evaluated by bidimen-cells were positive for type II collagen and chondroı ¨tin sional profilometry and revealed a rough surface for the sulfate, both hard markers of the chondrocyte phenoglass-ceramic compared to the plastic coverslips used as type. In addition, protein synthesis, alkaline phosphatase controls. Chondrocyte attachment was evaluated by measur-activity, and proteoglycan production were found to ining the number of attached cells after one day of culture and crease gradually during the culture period with a pattern by morphological observations. Chondrocytes attached in similar to that observed on control cultures. These results great numbers to the material surface by means of focal demonstrate that the bioactive glass-ceramic tested in contacts containing vinculin and ␤1-integrin. Fluorescent this study appears to be a suitable substrate for in vitro labeling of actin and vimentin revealed a poor spread-chondrocyte attachment, differentiation, and matrix producing of chondrocytes on the bioactive glass-ceramic com-tion.