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Beaded matrices from cross-linked alginate for affinity and ion exchange chromatography of proteins

✍ Scribed by M. Grasselli; L. E. Diaz; O. Cascone

Book ID: 104644713
Publisher: Springer-Verlag
Year: 1993
Tongue: English
Weight: 435 KB
Volume: 7
Category: Article
ISSN: 0951-208X
DOI: 10.1007/bf00152617

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✦ Synopsis

Toobtainalawcost,beadedchromatographicmatrix,calciumalginatebeadswerecross-linked with epichlorohydtin, and calcium was removed by sodium citrate treatment. The cross-linking reaction fix obtaining stable beads was optimbi For pur&aticm of haemoglobin by ion exchange, crxos~liuked calcium-free alginate heads have a b of 60 mg/ml and a Kd of 0.02 mg/ml gel, while far af3?nity polygalacturonase pui&ation K&was 0.007 ml/g.

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