BCL6 can repress transcription from the human immunodeficiency virus type I promoter/enhancer region

✍ Scribed by Beverly W. Baron; Manisha Desai; L. Julia Baber; Leilani Paras; Qiang Zhang; Annamma Sadhu; Stephen Duguay; Giuseppina Nucifora; Timothy W. McKeithan; Nancy Zeleznik-Le

• Book ID: 101259999
• Publisher: John Wiley and Sons
• Year: 1997
• Tongue: English
• Weight: 138 KB
• Volume: 19
• Category: Article
• ISSN: 1045-2257
• DOI: 10.1002/(sici)1098-2264(199705)19:1<14::aid-gcc3>3.0.co;2-3

✦ Synopsis

The gene BCL6 encodes a zinc finger protein with similarities to transcription factors. We previously reported that a number of viral genomes, including human immunodeficiency virus type 1 (HIV-1), contain sequences which are similar to the BCL6 DNA-binding consensus in their promoter regions. Electrophoretic mobility shift assays showed that the full-length BCL6 protein extracted from transfected COS cells and a bacterially expressed truncated protein containing the BCL6 zinc fingers can bind specifically to DNA from the U3 promoter/enhancer region of HIV-1. Transient transfections were performed to analyze the effects of the BCL6 protein on luciferase expression driven by the HIV-1 long terminal repeat (LTR) sequences. Full-length BCL6 significantly repressed luciferase activity compared with multiple controls. We conclude that the BCL6 protein can bind to the HIV-1 promoter-enhancer region and contains a domain upstream of its zinc fingers that can repress transcription from the HIV-1 LTR. Genes Chromosom.