Bcl-xl-Specific antibody labels activated microglia associated with Alzheimer's disease and other pathological states
✍ Scribed by B. Drache; G.E. Diehl; K. Beyreuther; L.S. Perlmutter; G. König
- Book ID
- 102653397
- Publisher
- John Wiley and Sons
- Year
- 1997
- Tongue
- English
- Weight
- 546 KB
- Volume
- 47
- Category
- Article
- ISSN
- 0360-4012
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✦ Synopsis
This report describes the production of a monoclonal antibody raised against Bcl-xl, and includes an initial study of bcl-xl expression in neuropathology including
Alzheimer's disease (AD). Bcl-xl is a potent apoptotic inhibitor and is known to be the predominant Bcl-x isoform in brain. To examine the expression of bcl-xl in aged brain and neurodegenerative disease, we raised a Bcl-xl-specific monoclonal antibody. In aged human brain, the highest bcl-xl expression was observed in cerebellum. By immunohistochemistry, significant bcl-xl expression was detected in reactive microglia of patients with AD and other neurological diseases such as progressive supranuclear palsy. Bcl-xlpositive microglia frequently colocalized with b-amyloid plaques in AD and with activated astrocytes in non-AD and AD brains, suggesting a general role for Bcl-xl in regions of pathology. High levels of Bcl-xl protein might render microglia more resistant to cytotoxic environments such as areas of neurodegeneration and astrogliosis.