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Bcl-XL as a fusion protein for the high-level expression of membrane-associated proteins

✍ Scribed by Khang Thai; Jungyuen Choi; Carla M. Franzin; Francesca M. Marassi

Book ID
119824443
Publisher
Cold Spring Harbor Laboratory Press
Year
2005
Tongue
English
Weight
329 KB
Volume
14
Category
Article
ISSN
0961-8368

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The gene for the integral membrane protein glycophorin A (GPA) was cloned in frame to the 5Ј end of the antibiotic resistance gene, neomycin phosphotransferase II (NPT). Protein expression was achieved in Escherichia coli as well as in mammalian cells. In case of Chinese hamster ovary cells (CHO) th