BCL-6 EXPRESSION IN REACTIVE LYMPHOID TISSUE AND IN B-CELL NON-HODGKIN'S LYMPHOMAS

✍ Scribed by PITTALUGA, STEFANIA; AYOUBI, TORIK A. Y.; WLODARSKA, IWONA; STUL, MICHEL; CASSIMAN, JEAN-JACQUES; MECUCCI, CRISTINA; VAN DEN BERGHE, HERMAN; VAN DE VEN, WIM J. M.; DE WOLF-PEETERS, CHRIS

• Publisher: John Wiley and Sons
• Year: 1996
• Tongue: English
• Weight: 866 KB
• Volume: 179
• Category: Article
• ISSN: 0022-3417
• DOI: 10.1002/(sici)1096-9896(199606)179:2<145::aid-path565>3.0.co;2-1

✦ Synopsis

Chromosomal abnormalities involving 3q27 have recently been associated with diffuse large B-cell lymphomas and, less frequently, with follicular lymphomas. Molecular studies have led to the identification of the BCL-6LAZ-3 gene, located at 3q27 and coding for a putative zinc-finger protein that might act as a transcriptional regulator during cell differentiation and development. Rearrangement of BCL-6 results in truncation of the gene in its 5' portion, leaving the protein intact; a resultant deregulation of its expression has been hypothesized. In order to test this hypothesis, the expression of BCL-6 protein was investigated in human reactive lymphoid tissue and compared with a group of non-Hodgkin's lymphomas (NHLs) with or without 3q27 anomalies andlor BCL-6 gene rearrangement.

BCL-6 protein is consistently expressed in reactive lymphoid tissues, where it is restricted to the follicle centre. The protein is also widely expressed in N H L all follicular lymphomas tested showed a pattern of expression similar to the reactive B follicle, independently of the presence of BCL-6 gene rearrangement and/or 3q27 anomalies. In the diffuse large B-cell lymphomas, there was more variation in BCL-6 expression, but a correlation with 3q27 anomalies andlor BCL-6 rearrangement was not found. Deregulation of the BCL-6 gene did not result in an aberrant tissue expression as detected by immunohistochemistry.