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Batch- and continuous-culture studies of a methane-utilizing mixed culture

✍ Scribed by S. C. Lamb; J. C. Garver

Publisher
John Wiley and Sons
Year
1980
Tongue
English
Weight
942 KB
Volume
22
Category
Article
ISSN
0006-3592

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✦ Synopsis

Abstract

A methane‐utilizing mixed culture isolated from activated sludge by selective enrichment at 45°C was found to consist of three interacting species: a methaneutilizing bacterium, a citrate‐utilizing bacterium, and a methanol‐utilizing bacterium. All three species grew well at 45°C. Three different stable mixed cultures were reconstituted by various combinations of these pure cultures. The nutritional requirements and substrate ranges for each pure culture were determined. The nutritional requirements and substrate ranges for each pure culture were determined. The saturation constant for the methane‐utilizing bacterium on methane (K) and for the methanol‐utilizing bacterium on methanol (K) were 1.73 × 10^−6^M and 4.51 × 10^−7^M, respectively. The volumetric mass transfer coefficient for methane (K~L~a) was determined to be 65.6 hr^−1^.

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## Abstract Experiments are reported in which a mixed population of organisms was continuous cultured on phenol in a 1‐ to 2‐liter well‐mixed vessel. Steady state phenol concentrations were measured for a range of inlet concentrations from 100 to 800 mg/liter at various dilution rates. These result