Basic Principles of Protease-Catalyzed Peptide Bond Formation
✍ Scribed by Prof. Dr. Hans-Dieter Jakubke; Dr. Peter Kuhl; Dr. Andreas Könnecke
- Publisher
- John Wiley and Sons
- Year
- 1985
- Tongue
- English
- Weight
- 928 KB
- Volume
- 24
- Category
- Article
- ISSN
- 0044-8249
No coin nor oath required. For personal study only.
📜 SIMILAR VOLUMES
As a model reaction for peptide and bond formation, the s N 2 reactions between glycine and ammonia have been studied with and without amine catalysis: using ab initio molecular-orbital methods. For each of the catalyzed and uncatalyzed reactions, two reaction mechanisms have been examined: a two-s
Benzyloxycarbonyl-L-proline p-guanidinophenyl ester is an "inverse substrate" for trypsin; i.e., the cationic center is included in the leaving group instead of being in the acyl moiety. This substrate can be used in trypsin-catalyzed acyl-transfer reactions leading to the sythesis of Pro-Xaa peptid
## Abstract In order to determine the peptide bond specificity of calpain, human myelin basic protein (HMBP) was treated with purified calpain of bovine brain. Upon incubation, HMBP component I (HMBP‐1) was degraded into several peptides as demonstrated by sodium dodecy1 sulfate‐polyacrylamide gel