Basic evaluation of an immunoradiometric competitive inhibition assay for sialosyl-Tn antigen in sera in women assay conditions and normal values
✍ Scribed by Kanji Ryuko; Osamu Iwanari; Satoru Nakayama; Manabu Kitao; Kenji Karino; Jiro Endo
- Book ID
- 102670661
- Publisher
- John Wiley and Sons
- Year
- 1992
- Tongue
- English
- Weight
- 508 KB
- Volume
- 69
- Category
- Article
- ISSN
- 0008-543X
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✦ Synopsis
The assay conditions needed for an immunoradiometric competitive inhibition assay of sera in healthy women were studied using the monoclonal antibody TKHZ, which is known to recognize specifically sialosyl-a2,6-GalNAcal-0-serine/threonine (S-Tn) antigen, a mucinous cancer-related antigen. Stable results were obtained with an incubation time of 1.5 hours at room temperature. The intra-assay and inter-assay coefficients of variation were 3.27% and 3.07%, respectively. The mean (? standard deviation [SD]) levels of serum S-Tn in 602 healthy women was 21.2 U/ml (k8.4 U/ml). Values showed a normal logarithmic distribution. Although slightly higher levels were seen in postmenopausal compared with premenopausal women, the differences were not significant. The cutoff value of 41 U/ml was determined from data obtained in 602 healthy women; higher levels were observed in only 2%. Serum S-Tn levels were not strongly influenced by Lewis or ABO (H) blood type, smoking, pregnancy, parturition, or phase of menstrual cycle. The use of the S-Tn antigen as a tumor marker for various gynecologic cancers requires study. Cancer 1992;