Basic Confocal Microscopy

✍ Scribed by W. Gray (Jay) Jerome, Robert L. Price

Publisher: Springer International Publishing
Year: 2018
Tongue: English
Leaves: 371
Edition: 2nd ed.
Category: Library

No coin nor oath required. For personal study only.

✦ Synopsis

Basic Confocal Microscopy, Second Edition builds on the successful first edition by keeping the same format and reflecting relevant changes and recent developments in this still-burgeoning field. This format is based on the Confocal Microscopy Workshop that has been taught by several of the authors for nearly 20 years and remains a popular workshop for gaining basic skills in confocal microscopy. While much of the information concerning fluorescence and confocal microscopy that made the first edition a success has not changed in the six years since the book was first published, confocal imaging is an evolving field and recent advances in detector technology, operating software, tissue preparation and clearing, image analysis, and more have been updated to reflect this. Several of these advances are now considered routine in many laboratories, and others such as super resolution techniques built on confocal technology are becoming widely available.

✦ Table of Contents

Front Matter ....Pages i-xi
Introduction and Historical Perspective (Robert L. Price, W. Gray (Jay) Jerome)....Pages 1-20
The Theory of Fluorescence (W. Gray (Jay) Jerome)....Pages 21-36
Fluorescence Microscopy (W. Gray (Jay) Jerome, Robert L. Price)....Pages 37-71
Specimen Preparation (W. Gray (Jay) Jerome, John Fuseler, Caleb A. Padgett, Robert L. Price)....Pages 73-97
Labeling Considerations for Confocal Microscopy (R. M. Albrecht, J. A. Oliver)....Pages 99-134
Digital Imaging (W. Gray (Jay) Jerome)....Pages 135-153
Confocal Digital Image Capture (W. Gray (Jay) Jerome)....Pages 155-186
Types of Confocal Instruments: Basic Principles and Advantages and Disadvantages (John Fuseler, W. Gray (Jay) Jerome, Robert L. Price)....Pages 187-213
Setting the Confocal Microscope Operating Parameters (Amy E. Rowley, Anna M. Harper, Robert L. Price)....Pages 215-277
3D Reconstruction of Confocal Image Data (Thomas C. Trusk)....Pages 279-307
Analysis of Image Similarity and Relationship (Jesse Aaron, Teng-Leong Chew)....Pages 309-333
Ethics and Resources (W. Gray (Jay) Jerome, Robert L. Price)....Pages 335-342
Back Matter ....Pages 343-368

✦ Subjects

Life Sciences; Biological Microscopy; Cell Biology; Biomedical Engineering/Biotechnology; Biotechnology

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