Basic calcium phosphate crystals stimulate cell proliferation and collagenase message accumulation in cultured adult articular chondrocytes
✍ Scribed by Peter G. Mitchell; Janine A. Struve; Geraldine M. Mccarthy; Herman S. Cheung
- Book ID
- 102751860
- Publisher
- John Wiley and Sons
- Year
- 1992
- Tongue
- English
- Weight
- 851 KB
- Volume
- 35
- Category
- Article
- ISSN
- 0004-3591
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✦ Synopsis
Objective:
To investigate bcp (basic calcium phosphate) crystal-stimulated mitogenesis and collagenase gene transcription in primary cultures of porcine chondrocytes.
Methods:
The role of protein kinases in bcp crystal-stimulated dna synthesis was investigated using thymidine incorporation, kinase inhibitors, and protein kinase c (pkc) assays. northern blot analysis was used to determine the levels of collagenase c-fos and c-jun message.
Results:
Bcp crystals stimulated chondrocyte proliferation in a pkc-dependent manner. increased levels of collagenase message were preceded by an increased accumulation of c-fos, but not c-jun.
Conclusion:
Bcp crystals could contribute to the abnormal chondrocyte proliferation and collagenase secretion observed in some rheumatic diseases.