Base composition of intact nucleic acid oligomers

✍ Scribed by Stanley Mandeles; Charles R. Cantor

• Book ID: 102760186
• Publisher: Wiley (John Wiley & Sons)
• Year: 1966
• Tongue: English
• Weight: 555 KB
• Volume: 4
• Category: Article
• ISSN: 0006-3525
• DOI: 10.1002/bip.1966.360040706

✦ Synopsis

The base composition of intact, purified, oligonucleotides has been determined by comparing the absorption spectrum of each oligomer with calculated curves. The spectral curve of each oligomer was measured in 7 M urea a t three pH values and digitized a t 1mp intervals. The calculated curves consisted of the sums of the absorption spectra of mononucleotides in 7 M urea at the same pH's. A computer was used to make the comparisons and establish the best fit. Results are presented for nine of the ten possible dimer composition isomers; trimers from pancreatic and TI ribonuclease hydrolyzates of TMV-RNA; poly-A, poly-C, and poly-U; and intact tobacco mosaic virus-RNA.