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B[a]P-DNA adduct formation and induction of human epithelial lung cell transformation

✍ Scribed by Ben van Agen; Lou M. Maas; Ivo H. Zwingmann; Frederik-Jan Van Schooten; Jos C. S. Kleinjans

Publisher: John Wiley and Sons
Year: 1997
Tongue: English
Weight: 67 KB
Volume: 30
Category: Article
ISSN: 0893-6692
DOI: 10.1002/(sici)1098-2280(1997)30:3<287::aid-em6>3.0.co;2-i

No coin nor oath required. For personal study only.

✦ Synopsis

In this study we tested the suitability of the human linearly correlated with colony formation in AIG epithelial lung cell line BEAS-2B for in vitro studies and with the number of cells within individual coloof lung carcinogenesis. The human bronchial epi-nies but not the number of colonies in the CFE test. thelial lung cell line BEAS-2B, immortalized with an At higher B[a]P concentrations, the clonal expan-SV-40/Ad-12 hybrid virus construct, was treated for sion of cells in the CFE and the number of colonies 24 hours with five different concentrations of the in the AIG declined. Also, the number of micronuclei lung carcinogen benzo(a)pyrene (B[a]P) to assess increased with the formation of DNA adducts. the relationship between DNA adduct levels, cell

It is concluded that after 24 hours of incubation cycle distribution, micronuclei formation (MN), col-with 100 nM B[a]P, the formation of BPDE-DNA ony forming efficiency (CFE), and anchorage inde-adducts in the human epithelial lung cells BEAS-2B pendent growth (AIG).

results in maximal induction of cell transformation. There appeared to be a strong linear correlation Because of this correlation between DNA adduct between B[a]P concentration and DNA adduct for-formation and lung epithelial cell transformation, mation, but no difference in cell cycle distribution the BEAS-2B cells seem suitable for in vitro studies was observed after incubation with various concen-on lung carcinogens. Environ. Mol. Mutagen. trations of B[a]P. In the incubation range of 4 to 30:287 -292, 1997 ᭧ 1997 Wiley-Liss, Inc. 100 nM B[a]P, the number of DNA adducts was

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