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Bamboo germplasm screening with nuclear restriction fragment length polymorphisms

โœ Scribed by E. Friar; G. Kochert

Publisher
Springer
Year
1991
Tongue
English
Weight
611 KB
Volume
82
Category
Article
ISSN
0040-5752

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โœฆ Synopsis

Bamboo species are difficult to identify because flowering material is seldom available and taxonomy is of necessity based on vegetative characters. To evaluate the utility of restriction fragment length polymorphism (RFLP) analysis in bamboo systematics and germplasm screening, a library of random genomic probes from a Phyllostachys nigra PstI library was constructed. Probes from the library were used to screen bamboo germplasm consisting mostly of temperate bamboos of the genus Phyllostachys. RFLP variation was abundant, and species-specific patterns were readily obtained. Chloroplast DNA showed little variation among the bamboo accessions analyzed.

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