Purification and characterization of an
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Nicholas A. Flint; Kurt E. Amrein; Thomas Jascur; Paul Burn
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Article
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1994
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John Wiley and Sons
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English
⚖ 898 KB
The lymphocyte-specific, nonreceptor protein tyrosine kinase Lck has been purified from an Escherichia coli expression system using a monoclonal antibody column followed by dye-affinity chromatography. Polyacrylamide gel electrophoretic analysis of purified protein revealed a single 56 kDa band, ind