<p><span>This highly anticipated second edition details cutting-edge experimental approaches for bacteriophage research. This comprehensive volume details new and updated experimental approaches for isolating, formulating, and engineering bacteriophage medicinal products. Authored by leading experts
Bacteriophage Therapy: From Lab to Clinical Practice
✍ Scribed by Joana Azeredo, Sanna Sillankorva (eds.)
- Publisher
- Humana Press
- Year
- 2018
- Tongue
- English
- Leaves
- 300
- Series
- Methods in Molecular Biology 1693
- Edition
- 1
- Category
- Library
No coin nor oath required. For personal study only.
✦ Synopsis
This volume details the experimental approaches suitable for isolating and characterizing bacteriophages to formulating bacteriophage medicinal products and clinical application. Chapters guide readers through regulatory compliance and safety aspects of bacteriophage therapy. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.
Authoritative and cutting-edge, Bacteriophage Therapy: From Lab to Clinical Practiceaims to ensure successful results in the further study of this vital field.✦ Table of Contents
Front Matter ....Pages i-xi
Front Matter ....Pages 1-1
Isolation of Bacteriophages for Fastidious Bacteria (Shigenobu Matsuzaki, Jumpei Uchiyama, Iyo Takemura-Uchiyama, Takako Ujihara, Masanori Daibata)....Pages 3-10
Isolation of Bacteriophages of the Anaerobic Bacteria Bacteroides (Cristina García-Aljaro, Maite Muniesa, Juan Jofre)....Pages 11-22
Isolation of Bacteriophages for Clinically Relevant Bacteria (Sanna Sillankorva)....Pages 23-30
Front Matter ....Pages 31-31
In Vitro Activity of Bacteriophages Against Planktonic and Biofilm Populations Assessed by Flow Cytometry (Diana P. Pires, Luís D. R. Melo)....Pages 33-41
Observation of Bacteriophage Ultrastructure by Cryo-electron Microscopy (Ana Cuervo, José L. Carrascosa)....Pages 43-55
Bacteriophage Taxonomy: An Evolving Discipline (Igor Tolstoy, Andrew M. Kropinski, J. Rodney Brister)....Pages 57-71
Front Matter ....Pages 73-73
Determination of the Bacteriophage Host Range: Culture-Based Approach (Andrey V. Letarov, Eugene E. Kulikov)....Pages 75-84
Recovery and Characterization of Bacteria Resisting Infection by Lytic Bacteriophage (Libera Latino, Christine Pourcel)....Pages 85-98
Guidelines to Compose an Ideal Bacteriophage Cocktail (Maia Merabishvili, Jean-Paul Pirnay, Daniel De Vos)....Pages 99-110
Front Matter ....Pages 111-111
Interaction of Bacteriophages with Mammalian Cells (Zuzanna Kaźmierczak, Krystyna Dąbrowska)....Pages 113-122
In Vivo Bacteriophage Biodistribution (Nicolas Dufour, Raphaëlle Delattre, Laurent Debarbieux)....Pages 123-137
Interaction of Bacteriophages with the Immune System: Induction of Bacteriophage-Specific Antibodies (Krystyna Dąbrowska)....Pages 139-150
Bacteriophage Treatment of Infected Diabetic Foot Ulcers (Vera V. Morozova, Yulia N. Kozlova, Denis A. Ganichev, Nina V. Tikunova)....Pages 151-158
Compassionate Use of Bacteriophage Therapy for Foot Ulcer Treatment as an Effective Step for Moving Toward Clinical Trials (Randolph Fish, Elizabeth Kutter, Gordon Wheat, Bob Blasdel, Mzia Kutateladze, Sarah Kuhl)....Pages 159-170
Front Matter ....Pages 171-171
Bacteriophage Production in Bioreactors (Maryam Agboluaje, Dominic Sauvageau)....Pages 173-193
Computational Modeling of Bacteriophage Production for Process Optimization (Konrad Krysiak-Baltyn, Gregory J. O. Martin, Sally L. Gras)....Pages 195-218
Methods for Bacteriophage Preservation (Małgorzata B. Łobocka, Aleksandra Głowacka, Piotr Golec)....Pages 219-230
Front Matter ....Pages 231-231
Bacteriophage Production in Compliance with Regulatory Requirements (Jean-Paul Pirnay, Maia Merabishvili, Hilde Van Raemdonck, Daniel De Vos, Gilbert Verbeken)....Pages 233-252
Guidelines for Bacteriophage Product Certification (Alan Fauconnier)....Pages 253-268
Front Matter ....Pages 269-269
Nano/Micro Formulations for Bacteriophage Delivery (Pilar Cortés, Mary Cano-Sarabia, Joan Colom, Jennifer Otero, Daniel Maspoch, Montserrat Llagostera)....Pages 271-283
Synthetic Biology to Engineer Bacteriophage Genomes (Ana Rita Costa, Catarina Milho, Joana Azeredo, Diana Priscila Pires)....Pages 285-300
Back Matter ....Pages 301-303
✦ Subjects
Microbiology
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