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Bacteriophage T4 rIIB protein synthesis with a temperature-sensitive mutation in the rIIB initiation codon

✍ Scribed by Belin, Dominique

Publisher: Springer
Year: 1979
Tongue: English
Weight: 864 KB
Volume: 171
Category: Article
ISSN: 0026-8925
DOI: 10.1007/bf00274012

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✦ Synopsis

In protein synthesis, the incorporation of an N-terminal formylmethionine residue is directed by an initiation codon. The most frequently used codon is AUG, although initiation at GUG and UUG codons has also been observed. The HD263 mutation is an AUG to AUA change in the rIIB initiation codon. Evidence is presented here that wild type and HD263 rIIB proteins, whether synthesized in vivo or in vitro, have identical fmet peptides. It is concluded that translation began at the AUA mutant initiation codon in vitro and in phage T4 infected cells. In the in vitro translation system used in these studies, the rIIB protein synthesized at 25 degrees no longer contains the N-terminal formyl group whereas a large proportion of the formyl group is retained at 37 degrees.

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